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S100A4 mRNA expression in peritoneal fluid and peritoneal metastasis of gastric cancer

amSW, KimTY, cta1. OverexpressionofS100A4iscloselyrelatedtotheaggressivenessofgastriccanccr knock. APMIS, 2003,[link widoczny dla zalogowanych], l1l (5): 539 ~ 545llSchmidt - HansenB. OrnasD, GrigorianM, Cta1. ExtracellularS100A4 (mts1) sdrnulatesinvasivegrowthofmouseendothelialcellsandmodulatesMMP - 13matrixmetalloproteinaseactivity knock. Oncogene, 2004,23 (32): 5487 ~ 549512ZhangS, WangG, FemigDG, cta1. Interactionofmetastasis-in-ducingS100A4proteininvivobyfluorescencelifetimeimagingmicroscopy knock. EurBiophysJ, 2005,34 (1): 19 ~ 27l3 XU Hui-Min, CHEN Jun-qing, WANG Shu Bao. Subclinical peritoneal metastasis of gastric cancer detection and blocking therapy fields. Chinese Journal of Clinical Oncology, 2002,29 (2): 134 ~ 137 (2OO7-0l-l2 Received) (2OO7-04-04 Revised) (Yang Hongxin proofreading) Caspase3 a 398 in the NS induced apoptosis in Hela cervical cancer Yin-Ling Pu role of surgery Demin Weili Bei Guo Xiaopeng Clinical College of Wuhan University (Wuhan 430065) Keywords cyclooxygenase type Caspase3Bcl-2 apoptosis cyclooxygenase 2 (COX-2) were upregulated in many cancers, can induce tumorigenesis, invasion and metastasis 1_. Non-steroidal anti-inflammatory drugs (NSAIDs) can reduce the incidence of digestive system cancer and mortality i3], its anti-cancer mechanisms and inhibition of cyclooxygenase-2 related. COX-2 in chronic cervicitis, FIGO staging of cervical cancer, the expression of different pathological grades also increased in the trends], it selectively block or inhibit the activity of COX-2 may facilitate the prevention of cervical cancer and treatment. Experimental observation of selective COX2 inhibitors of the NS-398 in vitro Hela cervical cancer cells, and to explore its possible mechanism. 1 Materials and methods 1.1 Materials NS 398 was purchased from a Sigma. A mouse anti-human Bcl 2, Caspase3 monoclonal antibodies were purchased from SantaCruz company. Caspase3 activity kit was purchased from U.S. companies Jing (Shenzhen). 1.2 cell culture by conventional adherent culture Hela cervical cancer cell line, cell activity taking more than 98% of the cells for experiments. This project by the Education Department Foundation of Hubei Province (No.: D200511008) ① Tongji Hospital, Huazhong University of Science Obstetrics and Gynecology Corresponding author: Yin-Ling yinling65 @ yahoo. eom. eH1.3 for determination of cell cycle and apoptosis in the NS cells after a 398, made of 1X10 single cell suspension, washing the same set. Adding 200 ~ 1RNaseA (1g / L),[link widoczny dla zalogowanych], 37 ℃ water bath 30min, coupled with the dark reaction of PI dye 30min, the machine testing, Muhicycle software analysis hypodiploid peak. 1.4WesternBlot analysis of Bcl-2 and a 398 Caspase3 the NS cells after treatment by washing, centrifugation, pre-cooling of the cell lysate by adding 0.1ml lysis, centrifugation. Determination of protein content in the supernatant. Protein sequence of each group were taken electrophoresis, transfer film, closed. Each with an anti-Bcl 2, Caspase3 (in reference to B-actin antibody) were incubated at 4 ℃,[link widoczny dla zalogowanych], rinsed, and then secondary antibody (horseradish peroxidase labeled goat anti-rabbit IgG) shocks were incubated 2h, dropping brightening agent , X-ray exposure imaging,[link widoczny dla zalogowanych], scanning the content of the protein bands, repeated three times, and the mean value. 1.5Caspase3 activity detected by ActiveCaspase3ApoptosisKit seized ~ NCaspase3 activation, according to kit instructions. 1.6 statistical package statistical methods used SPSSl1.5 t-test analysis.

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