www.uggbootst.com Hemolymph of Penaeus monodon and

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Hemolymph of Penaeus monodon and determination of norfloxacin pharmacokinetics

0,[link widoczny dla zalogowanych],79 (11): 988ANALYTICALnETHoDoFNoRFLoXACTHEGIANTTIGERS Foot MP (PE?, 【C4E (DDD Ⅳ) HEMoLYMPHANDBR Ⅱ FSTUDYoNPHARMACoK canthal TICSFANGWen-hong,[link widoczny dla zalogowanych], SHAOJin-hua, SHIZhao-hongandYANGXian-shi (EastChinaSeaFisheriesResearchInstitute, ChineseAcademyofFisheriesSciences, Shanghai200090,[link widoczny dla zalogowanych], China) Abstract: ARP-HPLCmethodwasdevelopedtodeterminethenorfloxacinhemolymphconcentrationofthegianttigershrimp, Penaeusmonodon . HPLCanalysiswasperformedbyAgilent1100.ThecolumnwasAgilentZORBAXSB-C18.Themobilephaseconsistedofacetonitrile: phosphatebuffer (10mmol/Lsodiumphosphatemonobasicand5mmol/Lte-trabutylammoniumbromide) (14.5:85.5, V / V). Theflowrate0.5mLmin ~, theUVdetectorwassetat280nm, andthecolumntemperaturewas25oC.Thelinearrangeofnorfloxacinwas0.051-20.4ug / mL.therecoveryofnorfloxacinfromhemolymphWas96.49 ± 1.60%, andtheintra-dayandinter-dayprecisionwereunder3%. TheRP-HPLCmethodcouldbeadaptedtodeterminethenorfloxacinconcentrationinhemolymphandwasusedtobrieflystudythepharmaoki-neticsofnorfloxacinintheshrimp.Atwo-compartmentopenpharmacokineticmodelwasusedtodescribethenorfloxacinhemolymphconcentration-timeprofileandthepharmacokineticmodelwasco = 11.73 × e a +22.62 × e'0 · 98t.Themodel-derivedpharmacokineticparametersweregained . Thedistributionhalf-life (t1 / 2) andeliminationhalf-life (to1 / 2) were0.0635and0.612h, respectively; theareaundertheconcentrationversustimecurve (AUC), totalbodyclearance (CLs) andapparentvolumeofdistribution (Vd) were12.425ug. h / mL, 804.83mL / kg. hand710.35mL/kg.Keywords: Penaeusmorlodon; RP-HPLCmethod; Norfloxacin; Pharmacokinetics

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