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): SPD a 10Avp UV detector, SCL a 10Avp system controller, LC a 10Avp infusion pump. Ferulic acid reference substance by the Pharmaceutical and Biological Products available; Angelica provided by the stations in Liuzhou medicine; acetonitrile for chromatography pure; other reagents were analytical grade. 2 Experimental Methods and Results 2.1 Experimental design using orthogonal test method,[link widoczny dla zalogowanych], the content of ferulic acid as an index, select the soaking time, extraction time, extraction times, solvent as the study of the four factors from each of 58 - ( total 182) are three levels of the experiment (Table 1). The level of experimental factors in Table 1 Table 2.2 2.2.1 Determination of ferulic acid column chromatographic conditions: Inea-silCl8 column (4.6mm × 250mm, 5m); mobile phase: acetonitrile of water [40:60 ( v / v),[link widoczny dla zalogowanych], the phosphoric acid pH value to 2-3】; column temperature: 30 ~ C; flow rate: 0.8ml/min; detection wavelength: 320nm. 2.2.2 Preparation of standard curve, accurately weighed dried to constant weight amount of ferulic acid reference standard, plus 60% methanol solution into 0.052mg/ml, as a reference substance stock solution. Take the standard solutions, respectively,[link widoczny dla zalogowanych], in the above chromatographic conditions, sample 2. l, l, 7. l, 10tzl, 12.5tzl, ferulic acid, the sample volume () of the peak area (1) linear fit, the regression equation is: Y = 9544X a 100081. r = 0.99972.2.3 Preparation of the test solution and determination to take Angelica Pieces log, press L. (3) orthogonal table (Table 2) design of boiling. Take 10ml decoction, add methanol to volume 100ml, the solution was tested products, check tested sample solution centrifuged (3000r/rain), separate the supernatant, over 0.45 / ~ m membrane. Depicting 5tzl sample, record the peak area, calculated according to the above regression equation of ferulic acid, the results in Table 2. From Table 2 we can see that the factors that affect the content of ferulic acid primary and secondary order C> B> D> A, A3 【optimal level):. However, analysis of variance showed that, A, B, C, D four factors were not significant (P> 0.05),[link widoczny dla zalogowanych], and soak and soak 50min 40rain the extraction of ferulic acid changed little, so the choice of ferulic acid Boiling water most of Traditional Chinese Medicine in June 2005 Volume 28 No. 3 Table 2 Orthogonal test design for the extraction process and the results good: 8 times the amount of solvent, soaking 40rain, extraction 2h, extracted 3 times. 2.3 The orthogonal test verification tests in accordance with the optimum conditions for the verification test 3 times, extracts were measured by the content of ferulic acid. The results of ferulic acid content and the maximum close to the orthogonal test, the method is feasible. Table 4. Table 4 Test results verify the number of ferulic acid (g / g) 1230.4300.4520.4503 discussion of ferulic acid has been reported 3.1 unstable, easy to light and heat decomposition of case b, can be seen from the experimental data out boiling water extraction of ferulic acid, while maintaining a certain period of time micro-boiling, A of Traditional Chinese Medicine in June 2005 Volume 28 No. 3 Wei acid does not break down with time. Boiling water extraction that ferulic acid is feasible. 3.2 Optimization of technology of ferulic acid water boiling experiments have not been reported, this experiment can be for the development of new dosage form of the drug provide an experimental basis.

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