ghd hair VX2 rabbit lung model and biological char

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VX2 rabbit lung model and biological characteristics

-Atin immunohistochemistry. Sections under a microscope. Animal model of randomly selected specimens of 5 cases of electrical Fund i state, The (strong Duhon Xue, Li Chongyan,[link widoczny dla zalogowanych], Wen Shuang, Cao Youde, Fan Wei, Bai Jin, Wu Feng, SECOND MILITARY) re-Lung Chuang Division Second Affiliated Hospital of Qu Qian c) Table 13 Comparison of rabbits lung cancer model - Jane Hand Gen child microscope. Quickly remove the tumor mass, cut into lmm size. Immediately placed in 4% glutaraldehyde at 4 ℃ refrigerator fixed 2h, phosphate buffer saline solution} application (PBS) after washing with 1% osmium tetroxide fixed after 2h, graded ethanol and acetone dehydration, provide transparent epoxy resin 618 , embedded c (1 form a mechanism of ultrathin sections prepared by semi-thin section, the positioning. the system of the thin film Ke, uranyl acetate and lead nitrate stain, H.600 electron microscopy. radiography. Second,[link widoczny dla zalogowanych], the results l_ general observation: suspension injection of l0 ~ 28d, sings into the tumor in situ was 10 096 (5 / 5), tumor diameter was 3.0 ~ 10,4 m. The number of tumor 6 ~ n months, was disseminated growth pattern, chest wall, diaphragm and lymph nodes of tumor growth Asr door. killed one, the other four rabbits, the average survival time (40 ± 4) d. mass implantation method l0 ~ 28d. orthotopic lung tumor rate 100% (20/20), tumor diameter was 45 ~ l2lmm, only a limited lung tumors were killed 5 Keratin immunohistochemistry. Su Su Yi eosin technique (HE) staining was observed see hilar lymph nodes tumor cell growth. 15 experiments, the average survival time for free (55 ± 5) d. nodules implanted flow method to install closed-I l0 ~ 28d. orthotopic lung tumor rate was 93.3% (14/15) tumor diameter was 44 ~ l13rean, only one in situ lung cancer, the growth of hilar lymph nodes, 80% (12/15) had pleural empyema rabbit. Typical model shown in color Doppler after 10d lower lobe 0 lung .8 to 1.0 twenty n the shadow of the size of ellipsoid, echogenic. x-ray see the lower right chest has a round spot l4 rabbits, the average survival time (26 ± 5) d. tumor shape oval or irregular shaped, hard. no obvious capsule, gray fish-shaped cut surface,[link widoczny dla zalogowanych], the three shown in table 1.2. morphological and electron microscopy: optical microscope the size of cancer nests different shape, we can see a bead of . cancer rowed, irregular, coarse chromatin, mitosis and more large nucleoli. electron microscopy were spindle or polygonal cells. edges / cytoplasm ratio increased. neighboring cells back to the bridge embedded,[link widoczny dla zalogowanych], fewer desmosomes, cytoplasmic filaments were Cambodian tension within the mitochondria Golgi less. developed endoplasmic reticulum, free ribosomes, polysomes and more. nuclear sizes and irregular. chromatin rich. large nucleoli. squamous cell carcinoma cells. Third, the discussion of commonly used animal model of lung cancer tumor cells were injected subcutaneously in mice established. promise more than three spheres, etc. _l transplanted with tumor cells in mice ear skin down the establishment of animal models. These methods were high tumor, but the established tumor model does not reflect the characteristics of the tumor in situ. We used in this experiment, 3 methods have a higher rate of tumor formation. suspension of the tumor injection disseminated growth, with the suspension spillover is related to the average survival time (40 ± 4) d; No. I closed the model flow method of tumor is higher. thoracotomy but tough action complex, to solve the open After the collapse of the lungs caused by thoracic respiratory failure and death of animals of the same title, after closed thoracic drainage tube placement success is also critical. The surgery is not an open platform for profiles with the animal is infected or even the development of pus The direct cause of breast, also reduced the average survival time; mass incision was sutured directly after implantation spell, using sterile needles out of the chest residual gas, restore spontaneous breathing animals. while also co-pack 【c Ding surface, alkali less infection rate of this method into cancer (10,096), animals live longer on average. It is limited to the early lung growth and metastasis of human lung cancer metastasis model similar to the manner and strong stability. Therefore, this experiment established lung cancer is an ideal animal model. as saying lung cancer diagnosis, treatment provides a basis for experimental study.

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