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Enzymes and heat treatment, the adhesion was significantly reduced, and the adhesion of trypsin inhibitor has a dose effect. High concentrations of bovine serum albumin treatment had no effect on adhesion of bacterial suspension _gJ. A human colon cancer cell line CCL 187 cells in normal colorectal tissue is close to the higher degree of differentiation of cells. Ge Changhui other 【In] DM9227 Bifidobacterium strains with a CCI 187 small Received Date :2002-08-01 About Zhongshi Shun (1969 a). Male. In the Ph.D., in the intestinal microflora of 【Summary】 cell adhesion in a preliminary study, observation of environmental pH values and calcium concentrations on adhesion of bifidobacteria was found when the pH value is maintained at 6.0 to 7 .0, Ca. Concentration of 2mmol / L when the adhesion of the maximum. But there are tests that: With the decline in pH value, the adhesion of bifidobacteria increased, pH4.0 pH7.4 time when the adhesion is twice the number of shows that acidic environment is to promote the adhesion of Bifidobacterium by cable [63. Perez and De1 adhesion of Bifidobacterium, respectively, and their aggregation and hydrophobicity were studied, confirmed the adhesion of bifidobacteria and their aggregation ability and surface hydrophobicity of bacteria highly relevant, it can be divided into Bifidobacterium To have the adhesion force, self-curing plants and do not have the force of adhesion, since the condensate line, with its cohesive power, and high cell surface hydrophobicity of bifidobacteria has a higher adhesion; the other hand, adhesion of bifidobacteria were more Low [1]. 3 adhesin of Bifidobacterium adhesion properties of cable is present in the microbial pili,[link widoczny dla zalogowanych], cell wall, outer membrane proteins, flagella, capsule and small filamentous microbial adhesion to a class of closely related special material, the protein may be , peptides, glycoproteins, glycolipids and polysaccharides or simple sugar, is a kind of structure and function with a variety of diverse molecules. Bacteria, the adhesion mediated by the mother claims not only on the target bacterial cell adhesion, stimulate cell adhesion secondary signaling pathway; and can be blocked by competitive inhibition mechanisms such as the bacteria have a similar structure of the adhesion receptor activity . Adhesion of Bifidobacterium but few studies cords. In 1985, OpdenCamp other with \In vitro with human intestinal epithelial cell adhesion test, found that the combination of LTA with the amount of time and strain dependence, and its binding is reversible, and prove that LTA is mediated by adhesion of bifidobacteria in the intestinal epithelial cell adhesion molecule A. LTA can stimulate the intestinal epithelial cells to produce TNF-and other inflammatory factors, increased local inflammation, which does not apply to intestinal barrier failure and other diseases. In 1993, Bernet et al [14] with the Gram stain, direct study of the Bifidobacterium and cultured human colon adenocarcinoma cell line Caco-2 adhesion, the adhesion of Bifidobacterium components that may be a bacterial surface unstable protein substances, such substances are also present in their spent culture medium. Accordingly,[link widoczny dla zalogowanych], they developed a culture supernatant of freeze-dried composition containing viable agents, the clinical effect than simply viable agents for the gifted, but the secretory nature of adhesion molecules has not been elucidated. Zheng Yuejie applications such as ammonium sulfate precipitation,[link widoczny dla zalogowanych], Superdex 75 gel filtration and a Q-sepharose ion exchange chromatography from culture supernatant of Bifidobacterium strains 1027 extract, purified protein a molecular weight of 16kDa, by including instruments and other cell adhesion Identification of species of bifidobacteria can be mediated and negative off pathogenic E. coli (EPEC) and enterotoxigenic Escherichia coli (ETEC) adhesion to intestinal epithelial cells. Almost at the same time, Japanese scholars Fujiwara so that the culture supernatant of Bifidobacterium could inhibit the adhesion of EPEC, and in 1999, isolated from a protein molecular weight 52kda, such substances can inhibit the EPEC of GA1 (Gangliotetraosylceramide) adhesion [1. Suggest the existence of Bifidobacterium adhesion to secretory component cable, and the adhesive composition may be more than one cable. 4 Bifidobacterium adhesion receptors of addition to adhesins, the adhesion index adhesion receptor is necessary for microbial substances, must have the appropriate host cell adhesion receptors cable to complete the process of adhesion between the two . Cable adhesion receptors are generally located on the host mucosal epithelial cells, most of which is a fatty acid receptor binding sites of proteins or glycolipids. Adhesion structures of different types of bacterial ligands, in different host cells have different receptors,[link widoczny dla zalogowanych], adhesion sites are different. Adhesion of Bifidobacterium adhesin and its intestinal epithelial cell adhesion receptor specific binding process, but current

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