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Red peritoneal endometriosis lesions block cell culture

The more solid. 3.3.4 lesion success rate of the level of activity is closely related with the collection of tissue , we should choose with rich vascular lesions , tissue blocks containing small blood vessels ,[link widoczny dla zalogowanych], such as , their survival rate was significantly higher than that of small blood vessels of the tissue . 3.3.5 A simplified procedure is different from the digestion experiment culture ,[link widoczny dla zalogowanych], tissue culture without going through hydrolysis , filtration and other steps of the larger cell damage , so that hard-earned small amounts of tissue can be fully utilized , to ensure the greatest degree of Cell survival. 3.3.6 Growth of primary organizations of time and planting density,[link widoczny dla zalogowanych], planting density should be suitable for the block . Too dense growth of cells would lose the space 278 premature , premature fusion; too thin reduces the interaction between cells , cell growth slow significantly extended the time for cell growth . Ideal planting density is maintained between the various tissue distance of 0.5cm . 3.4 Immunocytochemistry VEGFR in cultured peritoneal cells of red lesions of the red lesions on the peritoneal cells in vitro Fit a 1,[link widoczny dla zalogowanych], KDR by immunocytochemistry eutopic and ectopic detected in cultured cells , Flt- 1 and KDR are expressed mainly in the glandular cells , stromal cells expressed less, which results of immunohistochemistry consistent . Preliminarily confirmed that the cultured tissue culture cells in peritoneal red lesions to maintain the biological characteristics of cells , the cells cultured by this method can be used for internal and red lesions of endometriosis peritoneal cells in vitro .

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