 Wysłany: Nie 1:46, 16 Sty 2011 Temat postu: www.uggbootst.com Hemolymph of Penaeus monodon and |
|
| ,www.uggbootst.com
Hemolymph of Penaeus monodon and determination of norfloxacin pharmacokinetics 0,uggs,79 (11): 988ANALYTICALnETHoDoFNoRFLoXACTHEGIANTTIGERS Foot MP (PE?, 【C4E (DDD Ⅳ) HEMoLYMPHANDBR Ⅱ FSTUDYoNPHARMACoK canthal TICSFANGWen-hong,uggs nederland, SHAOJin-hua, SHIZhao-hongandYANGXian-shi (EastChinaSeaFisheriesResearchInstitute, ChineseAcademyofFisheriesSciences, Shanghai200090,uggs nederland, China) Abstract: ARP-HPLCmethodwasdevelopedtodeterminethenorfloxacinhemolymphconcentrationofthegianttigershrimp, Penaeusmonodon . HPLCanalysiswasperformedbyAgilent1100.ThecolumnwasAgilentZORBAXSB-C18.Themobilephaseconsistedofacetonitrile: phosphatebuffer (10mmol/Lsodiumphosphatemonobasicand5mmol/Lte-trabutylammoniumbromide) (14.5:85.5, V / V). Theflowrate0.5mLmin ~, theUVdetectorwassetat280nm, andthecolumntemperaturewas25oC.Thelinearrangeofnorfloxacinwas0.051-20.4ug / mL.therecoveryofnorfloxacinfromhemolymphWas96.49 ± 1.60%, andtheintra-dayandinter-dayprecisionwereunder3%. TheRP-HPLCmethodcouldbeadaptedtodeterminethenorfloxacinconcentrationinhemolymphandwasusedtobrieflystudythepharmaoki-neticsofnorfloxacinintheshrimp.Atwo-compartmentopenpharmacokineticmodelwasusedtodescribethenorfloxacinhemolymphconcentration-timeprofileandthepharmacokineticmodelwasco = 11.73 × e a +22.62 × e'0 · 98t.Themodel-derivedpharmacokineticparametersweregained . Thedistributionhalf-life (t1 / 2) andeliminationhalf-life (to1 / 2) were0.0635and0.612h, respectively; theareaundertheconcentrationversustimecurve (AUC), totalbodyclearance (CLs) andapparentvolumeofdistribution (Vd) were12.425ug. h / mL, 804.83mL / kg. hand710.35mL/kg.Keywords: Penaeusmorlodon; RP-HPLCmethod; Norfloxacin; Pharmacokinetics 相关的主题文章： mbt shoes clearance Rat heart mitochondria, the dy uggs nederland S7 ribosomal protein gene sequence Voltage-gated sodium channels in neuropathic pain Role _2866 |
|
